GAIN medium is a single-step cell culture medium that can be used up to blastocyst stage
GAIN medium is a bicarbonate buffered medium optimized to provide an excellent in vitro environment for human gametes and embryos.
Why GAIN medium?
GAIN medium can be used for sperm preparation, oocyte incubation and fertilization. It is a single-step medium designed for continuous embryo culture from day 1 to expanded blastocyst stage. The medium can also be used for embryo transfer. Oocyte and embryo culture should be performed in a CO2 incubator.
The medium contains 0.35% human serum albumin (EMA, Ph Eur, USP, FDA approved), gentamicin and low concentrations of phenol red (pH indicator). Information about the composition of the product can be found in the material safety data sheet.
| pH 37°C – 5% CO2 | 7.20-7.45 |
| Osmolality | 270-290mOsm/kg |
| Sterility | Sterile |
| Endotoxine | < 0.25 EU/mL |
| Mouse embryo test | ≥ 80% blastocysts after 96h incubation |
| In-use stability | Can be used safely up to 7 days after opening, when sterile conditions are maintained and the products are stored at 2-8°C |
| Albumin | FDA (USA) and EMA (Europe) compliant |
GAIN010 : GAIN medium – 1x 10mL
GAIN020 : GAIN medium – 5x 20mL
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