Fructose is secreted by the seminal vesicles, which contribute the major part of the ejaculate volume. Deficient secretion of the seminal vesicles occurs in cases of hypo-androgenism or after infection of the seminal vesicles. In these cases the ejaculate volume is lower than normal and the fructose concentration is decreased.

In congenital agenesis of the seminal vesicles, which is commonly associated with congenital bilateral absence of the deferential ducts (CBAVD), the patient presents with azoospermia, low ejaculate volume, acidic semen pH, and there is no fructose in the seminal plasma.

The Fructose test has a 12 month shelf life from production date.