Viscous medium for easy sperm capture in ICSI procedures
Intra-cytoplasmatic sperm injection (ICSI) requires the capture of individual sperm cells in a glass pipette for injection into the oocyte. This procedure is facilitated by first immobilizing the sperm.
Why 10% PVP in FertiCult Flushing medium?
The most widely used method of immobilizing the sperm cells is by placing them in a viscous medium prior to nicking the tail to immobilize sperm completely.
Our Ph Eur grade PVP solution in HEPES buffered FertiCult Flushing medium is used in ICSI procedures in humans. The viscosity of our product is such that optimal slowing down of the spermatozoa is obtained for easy pick-up of individual cells.
Information about the composition of the product can be found in the material safety data sheet.
| pH | 7.2-7.9 (7.2-7.6 at product release) |
| Osmolality | 280-340 mOsm/kg (280-320mOsm/kg at product release) |
| Sterility | Sterile (SAL 10-3 |
| Viscosity | >170cP at 25°C |
| Endotoxin | <1.0 EU/mL |
| Mouse embryo assay | ≥80% blastocysts after 96h incubation |
| Albumin | FDA (USA) and EMA (Europe) compliant |
| In-use stability | Can be used safely up to 7 days after opening, when sterile conditions are maintained and the products are stored at 2-8°C |
PVP0.2S : PVP – 5 x 0.2mL
PVP1 : PVP – 1 x 1mL
Contact us about your FertiPro needs
We supply the full range of FertiPro products. Call us on 0114 224 2257 to discuss your order. 96% of our orders are despatched within 48 hours.